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ibidi GmbH multiwell silicon chamber
Multiwell Silicon Chamber, supplied by ibidi GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiwell+silicon+chamber/multiwell+silicon+chamber/pm36230893-91-44-47
Average 90 stars, based on 1 article reviews
multiwell silicon chamber - by Bioz Stars, 2026-09
90/100 stars

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Incubation:

Article Title: Hmo1 Protein Affects the Nucleosome Structure and Supports the Nucleosome Reorganization Activity of Yeast FACT.
Article Snippet: Single-particle FRET (spFRET) measurements were conducted with an LSM710ConfoCor3 confocal microscope (Carl Zeiss, Germany) as described previously [13]. .. Nucleosomes N13/91, N35/112, and N57/135 in buffer A were used at 1 nM, with Hmo1 (1.33 μM), Nhp6 (1.33 μM), and FACT (0.13 μM) added as indicated in each experiment, incubated for 10 min at 37 ◦C, then subjected to spFRET measurements in a multiwell silicon chamber (Ibidi GmbH, Germany) fixed on a cover glass. .. Pre-formed protein-nucleosome complexes were mixed with competitor DNA (10 mg/mL, salmon sperm, Sigma, USA) directly on the microscope stage, followed by spFRET measurements.

Article Title: Hmo1 Protein Affects the Nucleosome Structure and Supports the Nucleosome Reorganization Activity of Yeast FACT
Article Snippet: Single-particle FRET (spFRET) measurements were conducted with an LSM710-ConfoCor3 confocal microscope (Carl Zeiss, Germany) as described previously [ ]. .. Nucleosomes N13/91, N35/112, and N57/135 in buffer A were used at 1 nM, with Hmo1 (1.33 μM), Nhp6 (1.33 μM), and FACT (0.13 μM) added as indicated in each experiment, incubated for 10 min at 37 °C, then subjected to spFRET measurements in a multiwell silicon chamber (Ibidi GmbH, Germany) fixed on a cover glass. .. Pre-formed protein-nucleosome complexes were mixed with competitor DNA (10 mg/mL, salmon sperm, Sigma, USA) directly on the microscope stage, followed by spFRET measurements.



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